Abstract Detail

Phylogenomics

Wilson, Carol [1], Freyman, Will [2].

Phylogenomics subgenus Hermodactyloides, a small lineage of bulbous Iris that are mostly narrow endemics.

Iris subgenus Hermodactyloides has about 15 taxa that are distributed across the Caucasus and eastern Mediterranean Basin, two biodiversity hotspots. The subgenus comprises species that emerge and flower in early spring, often as snow recedes, and soon enter dormancy after storing food reserves in bulbs. Dormancy remains through the warm summer and cool winters typical of their steppe habitats. Four sections are recognized within the subgenus based mostly on leaf and bulb characters. Recent studies of Iris, based on plastid markers, indicated that subgenus Hermodactyloides is polyphyletic. Because phylogenetic studies focusing on the subgenus are lacking we have little knowledge of evolutionary relationships among taxa in subgenus Hermodactyloides and related lineages or the status of sections that are circumscribed in the subgenus. This study included 31 samples representing 14 taxa and one sample each of five outgroups. Nucleotide sequence data was gathered using skimming techniques for plastomes and mitochondrial genes and targeted sequencing for nuclear genes. Probes were developed to enrich 635 targeted nuclear genes that are potentially single or low copy in Iris. Results confirm that the subgenus is polyphyletic although most sections are monophyletic. Although infraspecific taxa are recognized in the widespread Caucasian species, I. reticulata, current classification does not accurately capture all of the genetic diversity present. Subgenus Hermodactyloides s.s. are likely to have originated in the Caucasus, which is also the center of current diversity with multiple dispersals to the eastern Mediterranean Basin. Patterns of data conflict within and among genomes is discussed in relation to patterns of endemism.

1 -
2 - University of Minnesota, Department of Ecology, Evolution and Behaviour, 1479 Gortner Ave, Suite 140 , St Paul, MN, 55108, USA

Keywords:
Biogeography
Endemism
genome skimming
Iris
Iridaceae
Targeted Sequence Capture
Next generation sequencing.

Presentation Type: Oral Paper
Session: 36, Phylogenomics III
Location: 107/Mayo Civic Center
Date: Wednesday, July 25th, 2018
Time: 11:15 AM
Number: 36012
Abstract ID:957
Candidate for Awards:None