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Abstract Detail

Hybrids and Hybridization

Persinger, Jessica [1], Thompson, Pamela [1], Cruzan, Mitchell [1].

Hybridization and species delimitation in an Oregon Ranunculus species complex.

Species may be rare because of historical range contraction or they may occupy stable but limited geographic ranges. The fate of range-limited species is of particular concern when they are hybridizing with more common species due to the potential of genetic swamping. Plants pose a well-known challenge to the biological species concepts because of their high rate of hybridization. We have observed a putative hybrid zone between a candidate threatened species, Ranunculus austro-oreganus, and its more common congener, Ranunculus occidentalis. We use phenotypic measurements, greenhouse crosses, and genomic methods to infer the historical processes that have led to contemporary species distributions and outcomes from introgression between these species. We found significant differences in leaf hair density and petal color between the two species and their putative hybrids. Conflictingly, interspecific, intraspecific, self, and backcrosses (n=250) over two generations have revealed that these species are not strongly reproductively isolated. Using targeted capture of whole chloroplast genomes we recovered 38 haplotypes from 49 SNPs across 29 populations using the newly developed CallHap pipeline. These results show high levels of haplotype sharing between the two species, indicating ongoing gene flow. Our future studies using Genotyping-by-Sequencing will help understand the history of the hybrid zone. This study will help us understand whether hybridization is a threat or a benefit to Ranunculus austro-oreganus.

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1 - Portland State University, Biology, 1719 SW 10th Avenue, SRTC Rm 246, Portland, OR, 97201, USA

population genomics
reproductive isolation
single nucleotide polymorphisms.

Presentation Type: Oral Paper
Session: 10, Hybrids and Hybridization
Location: 101/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 4:15 PM
Number: 10011
Abstract ID:848
Candidate for Awards:None

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