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Abstract Detail

Evolutionary Developmental Biology (Evo-Devo)

Zhang, Jingbo [1], Kramer, Elena [2], Davis, Charles [3], Zhang, Wenheng [4].

Early spatial shift in CYC2 expression implicated in the development of floral zygomorphy in Malpighiaceae.

Recent advances in our understanding of the molecular basis of floral development have demonstrated that the CYC2 lineage of the TCP gene family has been repeatedly recruited for regulating the development of floral zygomorphy across diverse eudicots clades. Along these lines, most efforts to date have documented differential patterns of late stage CYC2 expression in floral development. Our limited knowledge of the expression of CYC2s in the early stages of zygomorphic development, however, has greatly hindered our ability to understand the patterns and potential function underlying the evolution and development of floral zygomorphy. We previously demonstrated that two CYC2 paralogs in Malpighiaceae, CYC2A and CYC2B, are differentially expressed along the plane of floral symmetry suggesting that CYC2s likely play a role in the establishment of late stage floral zygomorphy in this family. Notably, the late stage expression of CYC2B is solely restricted to the single, upright banner petal. Furthermore, our SEM study indicated a unique process of zygomorphic development in Malpighiaceae. Thus, the late stage phenotype featuring a single banner petal is established from an earlier zygomorphic condition where two petals are arranged in the dorsal position. The nature and timing of the pattern of CYC2 expression that accompanies this shift from two to one dorsal petal has not been explored. We examined CYC2B expression using RNA in situ hybridization in an NW species Callaeum macropterum (DC.) D.M. Johnson. Our results indicated that CYC2B is expressed broadly in early initiating floral organs, which is distinctly different from the CYC2B expression in the single banner petal observed at late stages of floral development. These findings suggest that CYC2B expression may change during development in concert with the morphological shifts to result in the distinct zygomorphic floral form in mature flowers of Malpighiaceae. Examining more precisely when this shift of gene expression takes place during development is a focus of ongoing investigations.

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1 - Department Of Biology, Virginia Commonwealth University, 1000 West Cary Street, Life Science Building 336, Richmond, VA, 23284, United States
2 - Harvard University, Organismic and Evolutionary Biology, 16 Divinity Avenue, Biological Laboratories 1119, Cambridge, MA, 02138, USA
3 - Harvard University Herbaria, Organismic and Evolutionary Biology, 22 Divinity Avenue, Cambridge, Massachusetts, 02138, United States
4 - Virginia Commonwealth University, Department Of Biology, 1000 W Cary Street, Richmond, VA, 23284, United States

CYC2-like genes
Floral development
floral symmetry
RNAin situ hybridization.

Presentation Type: Poster
Session: P, Evolutionary Developmental Biology (Evo-Devo)
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PEV006
Abstract ID:829
Candidate for Awards:None

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