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Abstract Detail


Mims, Mitchell A. [1], Lopez, Sarah M. [1], Lloyd, Yamiya [1], Sheardy, Erin E. [1], Alhabib, Zahraa [1], Baker, Stokes S. [1].

Isolating bacteria that potentially support giant duckweed (Spirodela polyrhiza) nutrient absorption.

The goal of the research is to determine what bacteria allows aquatic plants to absorb nutrients such as organic phosphates. Thus, bacteria that grow on giant duckweed (Spirodela polyrhiza) were plated out on modified Schenk and Hildebrandt media that contained DNA as the sole phosphorus source. To obtain a broader collection of organisms, duckweed bacteria were also plated out onto tryptic soy agar plates. To identify the isolated strains, the bacteria were DNA barcoded. PCR primers that span Variable Regions three, four and five of the 16S rRNA genes were used to produce amplicons that were Sanger sequenced. The resulting sequences were queried to the National Center for Biotechnology Information (NCBI), 16S ribosomal gene database via Basic Local Alignment Search Tool (BLAST). Results showed that members of the genus Pseudomonas were prevalent. Members of this genus commonly synthesis antibiotics. Some have been shown to aid the growth of agricultural plants. Members of the Flavobacterium were also isolated. Some of the stains isolated have high homology to fish pathogens. A colony with high homology to Rhizobium radiobacter (formally referred to as Agrobacterium tumefaciens) was also isolated. Barcodes from additional isolates are being sequenced. To determine if these bacteria help duckweed with nutrient absorption, sterile cultures of duckweed will be inoculated with the pure bacteria stains and grown on defined media containing DNA, phytate, or tricalcium phosphate as the sole phosphorous source.

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1 - University of Detroit Mercy, Biology Dept., 4001 W. McNichols Rd., Detroit, MI, 48221, USA

Giant duckweed
Sprirodela polyrhiza
Rhizobium radiobacter
Nutrient uptake.

Presentation Type: Poster
Session: P, Ecology
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PEC020
Abstract ID:786
Candidate for Awards:Ecological Section Best Undergraduate Presentation Award

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