Abstract Detail

Population Genetics/Genomics

Diaz, Nicolas [1], Persinger, Jessica [2], Thompson, Pamela [3], Cruzan, Mitchell [4].

Ploidy-mediated seed dispersal in an intervarietal hybrid zone of Eriophyllum lanatum.

Eriophyllum lanatum, or Oregon sunshine, is an enigmatic species complex with a range extending from southern California to British Columbia, and as far east as Montana and Wyoming. This taxonomically challenging and phenotypically diverse group is comprised of geographically delineated varieties that intergrade at the interfaces of their ranges. Despite a recent taxonomic treatment, the role of whole genome duplication in shaping the evolution and ecology of this lineage remains undetermined. In 2008, J.S. Mooring described a contact zone between E. lanatum var. leucophyllum and var. achilleoides in Southern Oregon that consists of morphologically intermediate diploid, tetraploid, and hexaploid populations. Population structure and seed dispersal in this hybrid zone are influenced by reproductive isolation among cytotypes and self-incompatibility across cytotypes. Here, we used flow cytometry to clarify the diversity and distribution of cytyopes across 22 populations of E. lanatum in Southern Oregon. We pair this analysis of ploidy variation with a landscape genetic analysis of seed dispersal among these populations. Following a targeted capture and de novo chloroplast genome assembly, we used the recently developed CallHap pipeline to reconstruct haplotype frequencies from SNP data, and discovered 42 haplotypes across the study site. We then analyzed chloroplast haplotype frequencies across these populations to determine the influence of landscape features, including elevation, soil type, rivers, and canopy cover, on seed dispersal. We discuss cytotype diversity and phenotypic comparisons across these populations, and the relevance of ploidy variation in dispersal.

1 - Portland State University, Biology Department, 1719 SW 10th Avenue, SRTC Rm 246, Portland , OR, 97201, United States
2 - Portland State Univeristy, Biology Department, 1719 SW 10th Avenue, SRTC rm 246, Portland, OR, 97201, USA
3 - Portland State Univeristy, 1719 SW 10th Avenue, SRTC rm 246, Portland, OR, 97201, United States
4 - Portland State University, Department Of Biology, 1719 SW 10th Ave, SRTC Rm 246 - Biology, Portland, OR, 97201, United States

Keywords:
Polyploidy
Landscape genomics
seed dispersal
Plastid population genomics.

Presentation Type: Oral Paper
Session: 47, Population Genetics and Genomics II
Location: 101/Mayo Civic Center
Date: Wednesday, July 25th, 2018
Time: 2:30 PM
Number: 47005
Abstract ID:764
Candidate for Awards:None