| Abstract Detail
Pteridology Liu, Shanshan [1], Wang , Zhen [2], Wang, Ting [3], Su, Yingjuan [1]. Chloroplast Comparative Genomics in Three Ferns Reveals Architectural Feature and New Insights into the Phylogeny of Neolepisorus and Phymatosorus (Polypodiaceae). Neolepisorus and Phymatosorus are two important genera in Polypodiaceae, which also occur great controversies in the classification. To further investigate their phylogenetic relationships, we first sequenced whole chloroplast genomes of N. fortunei, N. ovatus, and P. cuspidatus and compared their plastome structure features based on Illumina sequencing technology. The results indicated that the three fern cp genomes were typical quadripartite structure and high-conserved. The genome size ranged from 151,915 to 152,161 bp with same gene number and gene order. More than 40 cpSSRs were separately identified in the three ferns. Their majority were mononucleotides with C/G and distributed in non-coding regions. Six highly divergent regions (rps16 intron, psbK-psbI, trnS-ycf12, psbM-petN, trnV intron and rpl32-trnP) were detected among the three plastomes and would be very powerful molecular markers in phylogenetic analyses. The further phylogenetic investigation based the three datasets revealed new insights into the phylogeny of Neolepisorus and Phymatosorus. Neolepisorus fortunei was closer to P. cuspidatus than N. ovatus. Possible reasons lie in the trnR-UCG absence in P. cuspidatus and the 470-bp rps16 intron loss. In the future, it is necessary to sequence more chloroplast genomes of Neolepisorus and microsoroid ferns to deeply survey their phylogenetics.
Corresponding author: Ting Wang: tingwang@scau.edu.cn, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China; Yingjuan Su: suyj@mail.sysu.edu.cn, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
This work was supported by the National Natural Science Foundation of China [31370364, 31570652, 31670200, and 31770587], the Natural Science Foundation of Guangdong Province, China [2016A030313320 and 2017A030313122], Science and Technology Planning Project of Guangdong Province, China [2017A030303007], Project of Department of Science and Technology of Shenzhen City, Guangdong, China [JCYJ20160425165447211 and JCYJ20170413155402977], and Science and Technology Planning Project of Guangzhou City, China [201804010389].
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1 - Sun Yat-Sen University
2 - Nanjing Agricultural University
3 - South China Agricultural University
Keywords:
none specified
Presentation Type: Oral Paper
Session: 15, Pteridology II
Location: 108/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 4:30 PM
Number: 15004
Abstract ID:733
Candidate for Awards:None |
