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Abstract Detail

Population Genetics/Genomics

Prochazka, Jolene [1], Gross, Briana L. [1].

Clonal structure and genetic diversity of Vaccinium vitis-idaea in Minnesota.

Developing an understanding of the levels of diversity present at stages across the domestication continuum is key to eventually retaining or introducing diversity into domesticated plant lineages. While this goal is critical for both annual and perennial crops, there are special challenges associated with achieving this for clonal crops and their wild relatives. This is because in species capable of asexual reproduction, it is necessary to first differentiate between clonal ramets and unique genets before measuring genetic diversity. Here, we assessed the clonal structure and genetic diversity in wild populations of lingonberry (Vaccinium vitis-idaea), a domesticated species that utilizes both sexual and clonal modes of reproduction in the wild and under cultivation. This survey of V. vitis-idaea in northern Minnesota included 222 individual samples across 15 populations, assayed at 18 nuclear microsatellite loci. Five of the fifteen populations were found consist of only two clones each, while other populations maintained a higher number of unique individuals. He across all samples was 0.517 (Table 1) while Ho was higher, at 0.567, with a negative but nonsignificant value of FIS (-0.0970.038) across populations. Most of the variation was found between individuals (84%), with a moderate amount of variation between populations (17%), consistent with low levels of population structure at this geographical range. This survey has revealed the various survival strategies of V. vitis-idaea, a species with a mixed mating system. Genetic diversity assessments of wild perennial species, like V. vitis-idaea, can be used to support future breeding and conservation efforts.

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1 - University Of Minnesota Duluth, Department Of Biology, 207 Swenson Science Building, 1035 Kirby Drive, Duluth, MN, 55812, United States

Crop wild relatives
perennial crop
Genetic diversity
Clonal reproduction.

Presentation Type: Oral Paper
Session: 38, Population Genetics and Genomics I
Location: 101/Mayo Civic Center
Date: Wednesday, July 25th, 2018
Time: 9:15 AM
Number: 38006
Abstract ID:645
Candidate for Awards:Margaret Menzel Award

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