Abstract Detail

Crops and Wild Relatives

Onuminya, Temitope [1], Ogundipe, Oluwatoyin [2], Lawal, Oyewole [1].

Morphological Characterization and Genetic Diversity Studies on Accessions on Cowpea from Ten African Countries.

Molecular and morphological characterisations are critical tools in assessing the level of genetic diversity and the presence of variations in some traits. In this study, ten genotypes of cowpea (Vigna unguiculata (L) Walp) originating from different countries in Africa were obtained from IITA. The genotypes were planted in a randomized complete block design with four replicates. Morphological characters of seedlings were assessed following the International Board for Plant and Genetic Resource cowpea descriptor list, both quantitative and qualitative characters were evaluated. Days to first flowering range between 54-95days while the days to first ripe pod range between 67-113days. Peduncle length measurement ranges between 1.87-15.5cm while the pod length range between 8.63-18.83. Morphological relationship among the genotypes showed a dendogram with four clusters. DNA samples were successfully extracted from ten genotypes with concentration ranging from 339-923ng/µl and optical density of 1.20-1.87. All the samples were amplified by PCR using six SSR markers (SSR27BY, SSR19BY, SSR10BY, SSR9BY, SSR8BY and SSR21BY) however, only three SSR markers showed amplification. A total of 7 alleles were observed while the PIC ranges from 0.868 to 0.992. Molecular relationship among the genotypes showed a dendogram with two clusters and one outgroup. This study revealed low genetic diversity among the accessions and a relatively high variation based on morphological characters.

1 - University Of Lagos, Department Of Botany, Akoka Yaba, Lagos, 23401, Nigeria
2 - University of Lagos, Department of Botany, Akoka, Yaba, Lagos, 23401, Nigeria

Keywords:
Cowpea
Africa
Genetic diversity.

Presentation Type: Poster
Session: P, Crops and Wild Relatives
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCW001
Abstract ID:57
Candidate for Awards:None