Abstract Detail

Applications of Plant Genomics

Lim, Chae Eun [1], Park, Suhyeon [2], Lee, Byoung-Yoon [1], Kim, Sangtae [2].

Target-enrichment NGS sequencing data provided full resolution for identifying medicinally important Korean Artemisia taxa.

Artemisia, containing more than 500 species in world-wide, is one of the largest genus in the Asteraceae. It is well known for pharmacy and medicine in Asia since their rich accumulation of essential oils and terpenoids. Their morphological identification has been challenged in some taxa because of insufficient diagnostic characters and potential natural hybridization. Furthermore, standard plant barcoding markers such as matK, rbcL, trnH-psbA, and ITS did not provide full resolution to identify them because they showed very low genetic divergence rate. Recently, massive sequencing using target-enrichment (also called Hyb-Seq) NGS has been developed to solve many taxonomic problems with comparing a large number of single-copy genes. In this study, Hyb-Seq was performed on 159 samples(105 samples identified by experts and 54 unidentified samples) in 18 taxa including medicinal plants such as A. argyi, A. indica, and A. capillaris. About 46Gbp of raw data were generated and this was about 625X coverage per a sample. After the filtering of>70% of sequence coverage regions, 207Kbp of comparable sites were recognized. A Maximum-likelihood tree provided 100% resolution including infraspecific taxa and assigned unidentified samples into relevant clades represented by each species. Synapomorphic characters of each clade were recognized by the analysis of evolutionary character reconstruction. DNA sites having high CI values were recognized and they'll be used for the development of genome-based marker sets in order to facilitate molecular identification in the future. This study will provide the technological base for the quality control of industrial use and utilization of Artemisia.

1 - National Institute of Biological Resources, Plant Resource Division, Environmental Research Complex, Hwangyeong-ro 42, Seo-gu, Incheon, 22689, Korea
2 - Sungshin University, Department of Biology, 55, Dobong-ro 76ga-gil, Gangbuk-gu, Seoul, 01133, Republic of Korea

Keywords:
Artemisia
target enrichment
medicinal plant
genome-based marker.

Presentation Type: Colloquium Presentations
Session: C13, Applications of Plant Genomics
Location: 104/Mayo Civic Center
Date: Wednesday, July 25th, 2018
Time: 2:30 PM
Number: C13004
Abstract ID:568
Candidate for Awards:None