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Straub, Shannon [1], Boutte, Julien [1], Bailey, Donovan [2], Fishbein, Mark [3], Liston, Aaron [4].

Introduction to Next Generation Sequencing.

Next generation sequencing (NGS) describes technologies that produce millions to billions of base pairs of sequence data per experiment. The low cost of these technologies allows an increasing number of biologists to incorporate genome-scale sequencing into their research. The goal of this workshop is to introduce participants to the essentials of NGS, so that they can develop and initiate their own projects in phylogenetics, population genetics, and gene expression using this technology. In the morning, we will describe the available technologies, applications, and protocols for sample preparation and sequencing. We will also describe options for computational infrastructure and analytical pipelines. In the afternoon, participants will then have an opportunity to work with short read data from the Illumina platform and long read data from the PacBio platform. Topics covered during the hands-on portion will include plastome sequence assembly and probe design for nuclear gene target enrichment (Hyb-Seq). A laptop computer is required. The workshop fee includes lunch, coffee breaks, internet access, and power strips.

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1 - Hobart and William Smith Colleges, Department of Biology, 300 Pulteney St., Geneva, NY, 14456, USA
2 - New Mexico State University, Department of Biology, Las Cruces, NM, 88003, USA
3 - Oklahoma State University, Department of Plant Biology, Ecology, and Evolution, 301 Physical Sciences, Stillwater, OK, 74078, USA
4 - Oregon State University, Department of Botany and Plant Pathology, Cordley Hall, Corvallis, OR, 97331, USA

Next generation sequencing

Presentation Type: Workshop
Session: W03, Introduction to Next Generation Sequencing
Location: 110/Mayo Civic Center
Date: Sunday, July 22nd, 2018
Time: 8:00 AM
Number: W03001
Abstract ID:50
Candidate for Awards:None

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