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Abstract Detail



Comparative Genomics/Transcriptomics

Kenny, Cecile [1], Doyle, Jeff [2], Coate, Jeremy [3].

Exploring genetic mechanisms of polyploidy-induced salinity tolerance in Arabidopsis thaliana.

Polyploidy is correlated with enhanced salinity tolerance as evidenced by smaller reductions in biomass and seed yield under moderate salt stress and prolonged survival under severe salt stress. Reciprocal grafting experiments in Arabidopsis thaliana have shown that this tolerance is a root-specific phenotype, but the molecular mechanisms for this phenotype remain unresolved. To address this question, we are using RNA-Seq to compare transcriptional responses to salt stress in isogenic diploid/synthetic tetraploid pairs of Arabidopsis. These include diploid/tetraploid pairs in accessions that vary in level of salt tolerance at the diploid level, as well as in mutant lines of Columbia-0 (Col-0) defective for known salt-tolerance loci. Preliminary studies confirm that polyploidy improves survival and seed yield under salt stress across natural accessions. Here, equivalent data for diploid/tetraploid pairs in mutant backgrounds, as well as transcript profiling data, will be presented and their implications for the mechanisms of polypoid-induced salt tolerance will be discussed.


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1 - Reed College, 3203 SE Woodstock Blvd, Portland, Oregon, 97202, United States
2 - Cornell University, School Of Integrative Plant Science, Plant Breeding & Genetics Section, 240 Emerson Hall, Ithaca, NY, 14853, United States
3 - Reed College, Biology, 3203 SE Woodstock Blvd., Portland, OR, 97202, United States

Keywords:
Polyploidy
salt stress
RNA-Seq.

Presentation Type: Oral Paper
Session: 21, Comparative Genomics and Transcriptomics I
Location: 102/Mayo Civic Center
Date: Tuesday, July 24th, 2018
Time: 10:45 AM
Number: 21011
Abstract ID:494
Candidate for Awards:None


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