| Abstract Detail
Applications of Plant Genomics Braukmann, Thomas [1], Ivanova, Natalia [1], Kuzmina, Maria [1], Borisenko, Alex [1], Zakharov, Evgeny [1]. Biocomplexity of Herbal Supplements and DNA-based Authentication. Recent advances in DNA-based authentication have enabled fast and sensitive detection of DNA sources in herbal supplements. This is now utilized by some manufacturers for quality assurance of raw plant materials, final products, and contamination control during production. One of the common approaches, DNA barcoding, heavily relies on Sanger sequencing. However, stochastic amplification of multiple DNA sources that are often present even in single-source supplements renders Sanger results non-interpretable or non-reproducible, hence indicating strong need for NGS-based methods. While advances in NGS technology enable rapid and sensitive detection of DNA in complex mixtures, such results should be interpreted from a biocomplexity perspective. Aside from intended or non-intended substitution, possible cross-contamination with trace plant or fungal DNA can occur at any stage during growing, harvesting, manufacturing, handling or laboratory analysis of plant material. Detection of such non-target DNA is not necessarily indicative of technological flaws or deliberate adulteration and such results should be interpreted with caution. Diversity of fungi in herbal supplements is determined by a combination of pathogenic, saprophytic, endophytic and mycorrhizal fungi naturally associated with live plant material, and strains involved in the fermentation during manufacturing of bioactive components. Although this entire spectrum can be easily detected by NGS, interpretation of test results should focus on potential mycotoxin-producing fungi and human pathogens. While embracing this biocomplexity we are developing SNP-based diagnostic systems for selected species of interest by targeting multiple variable regions in chloroplast and nuclear DNA and propose NGS-facilitated detection method as a standardized tool for authentication of herbal supplements. Because manufacturing of extracts leads to DNA degradation or loss, quality control should utilize synergetic approach targeting both bioactive components (HPLC-MS) and DNA.
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Related Links:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0156426
1 - University of Guelph, Centre for Biodiversity Genomics, Biodiversity Institute of Ontario, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada
Keywords:
biocomplexity
NGS
herbal supplements
plant-fungus interaction
plant-plant interactions.
Presentation Type: Colloquium Presentations
Session: C13, Applications of Plant Genomics
Location: 104/Mayo Civic Center
Date: Wednesday, July 25th, 2018
Time: 2:15 PM
Number: C13003
Abstract ID:468
Candidate for Awards:None |
