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Abstract Detail

Comparative Genomics/Transcriptomics

Lawrence , Amelia H [1], Kinser, Taliesin [2], Puzey, Joshua [3].

Methylation repatterning in the endosperm and embryo of interploid Mimulus hybrids.

Plant hybridization offers a unique window to explore genomic interactions between divergent genomes. One potential manifestation of interactions between genomes in hybrid individuals is methylation repatterning. To understand the impact the interploid hybridization has on methylation repatterning we utilized Mimulus as a model system. Mimulus is useful because it has species of varying ploidies and its genome is fully sequenced. Because the endosperm and embryo are the first areas where these genomes interact, we examine methylation repatterning in the context of these tissues. Using Mimulus we examined endosperm and embryo methylation in reciprocal crosses between M. guttatus (2n) and M.luteus (4n) (2n x 4n and 4n x 2n) as well as parental crosses (2n x 2n and 4n x 4n). Overall, we find that all types of methylation (CG, CHH, CHG) are significantly repatterned in the hybrid endosperm and embryo. These results have broad implications for understanding genomic imprinting, seed development, and hybrid incompatibility.

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1 - College of William & Mary, Biology, 540 Landrum Dr., 110 Sadler Center, Williamsburg, Virginia, 23185, United States
2 - College of William & Mary, 540 Landrum Dr., Williamsburg, Virginia, 23185, United States
3 - College Of William And Mary, Biology Dept., 540 Landrum Dr., Williamsburg, VA, 23185, United States


Presentation Type: Poster
Session: P, Comparative Genomics and Transcriptomics
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PGT008
Abstract ID:282
Candidate for Awards:Genetics Section Poster Award

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