| Abstract Detail
Revolutionizing systematics: Herbaria in the Genomics Age Beck, James [1], Jordon-Thaden, Ingrid [2], Rushworth, Catherine [3], Windham, Michael [4], Diaz, Nicolas [5], Cantley, Jason [6], Martine, Christopher [7], Rothfels, Carl [3]. I fall to pieces: RADseq techniques can be applied to herbarium-derived DNAs of varying quality. Although herbarium genomics studies are beginning to include restriction site-associated DNA (RADseq) techniques, concerns remain regarding the link between herbarium DNA degradation and data recovery. This is particularly true for RADseq, since highly-sheared DNAs could include relatively few intact fragments flanked by appropriate cut sites. Here we apply a basic double-digest restriction-site associated DNA Sequencing (ddRADseq) protocol to multiplexed samples from four genera (Boechera, Draba, Ilex, and Solidago). Herbarium-derived DNAs comprised 60% of the sample set, spanning collection dates from 1883-2014. We demonstrate that RADseq is effective for generating large phylogenetically informative datasets from herbarium specimens, and the relationship between specimen age, DNA degradation, and locus recovery is presented. The potential for a cost-effective RADseq protocol for sample sets comprising multiple lineages and tissue types is discussed. Log in to add this item to your schedule
1 - Wichita State University, Biology, 1845 Fairmount, Box 26, Wichita, KS, 67260, United States 2 - University of Wisconsin, Department of Botany, 430 Lincoln Drive, Madison, WI, 53706 3 - University of California, Berkeley, University and Jepson Herbaria and Department of Integrative Biology, Berkeley, CA, 94720, USA 4 - Duke University, Department Of Biology, Box 90338, Durham, NC, 27708, United States 5 - Portland State University, Department of Biology, 1719 SW 10th Avenue, Portland, OR, 97201, USA 6 - San Francisco State University, Department of Biology, 1600 Holloway Avenue, San Francisco, CA, 94132, USA 7 - Bucknell University, Department of Biology, 1 Dent Drive, Lewisburg, PA, 17837, USA
Keywords: herbarium degraded DNA RADseq Phylogenomics Draba Solidago Ilex Boechera.
Presentation Type: Colloquium Presentations Session: C10, Revolutionizing Systematics: Herbaria in the Genomics Age Location: 103/Mayo Civic Center Date: Wednesday, July 25th, 2018 Time: 8:45 AM Number: C10004 Abstract ID:233 Candidate for Awards:None |