| Abstract Detail
Comparative Genomics/Transcriptomics Song, Michael [1], Potter, Barney [2], Doyle, Jeff [3], Coate, Jeremy [4]. Quantifying transcriptome size variation and dosage responses immediately following ploidy change in Arabidopsis thaliana. It is now widely considered that all vascular plants have experienced ancient whole genome duplications (WGDs) and subsequent genome reductions. There are biases in the patterns of gene retention and loss based on the types of duplications experienced and the types of genes involved. The Dosage Balance Hypothesis (DBH) has been proposed to account for the observation that classes of genes which retain duplicates originating from WGDs will often have proportionately fewer genes that derive from small-scale duplications. Although dosage responses have been measured for allopolyploids in young natural populations, very little is known about the effects of WGD on transcriptome size (the number of transcripts per cell) and therefore on individual gene dosage responses. We sequenced the transcriptomes of two synthetic autopolyploid accessions of Arabidopsis thaliana and their diploid progenitors to estimate transcriptome size and gene dosage responses immediately following ploidy change. Our preliminary results are consistent with the DBH and illustrate that there are immediate transcriptional responses to polyploidy—induced gene dosage increase that are consistent across genotypes at the level of gene ontology and metabolic networks, but vary among the individual genes expressed. Log in to add this item to your schedule
1 - University of California, Berkeley, UC Berkeley, Berkeley, CA, 94720, United States 2 - Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 3 - Cornell University, School Of Integrative Plant Science, Plant Breeding & Genetics Section, 240 Emerson Hall, Ithaca, NY, 14853, United States 4 - Reed College, Biology, 3203 SE Woodstock Blvd., Portland, OR, 97202, United States
Keywords: Polyploidy dosage transcriptomes whole genome duplication Evolution.
Presentation Type: Oral Paper Session: 21, Comparative Genomics and Transcriptomics I Location: 102/Mayo Civic Center Date: Tuesday, July 24th, 2018 Time: 10:30 AM Number: 21010 Abstract ID:210 Candidate for Awards:Margaret Menzel Award |