Abstract Detail

Functional Genetics/Genomics

Wilson, Rachel [1], Michaels, Helen [2].

Genetic Basis of Seed Coat Polymorphisms in Lupinus perennis.

Multigenetic traits, such as seed coat phenotypes, are poorly understood in domesticated plants. This gap in knowledge can be filled by studies in wild relatives. I am investigating if mutations in the structural genes of the Anthocyanin Biosynthetic Pathway (ABP) lead to seed coat polymorphisms in Lupinus perennis. Legumes have been artificially selected for biochemical traits that are often correlated with color, size, taste, and chemical composition. For example, sweetness of seeds has been correlated to low alkaloid production. Lupin, a type of legume, is used as livestock feed but is beginning to gain popularity as a health food due to the high protein levels (30-40%) in the seeds. As a cover crop, Lupin creates root nodes for rhizobium bacteria to colonize that convert atmospheric nitrogen to biologically usable nitrogen. Also, Lupinus perennis (wild relative) is a host plant for endangered butterflies like the Karner Blue (Lycaeides melissa samuelis) and correlated biochemistry of leaf tissue can impact restoration efforts, while Lupinus angustifolius (domesticated) has recently had its genome sequenced. The seed coat is the outermost layer protecting the seed, and is composed of maternal tissue. Seed coats are known to control dormancy, predation, and microbe interactions. Polymorphic seeds have been documented in many plants such examples are chickpea and soybean. Accordingly, phenotypic differences have been shown to relate to differences in chemical composition. Also, it is shown that genistein, naringenin, and malvidin (products of the ABP) are in higher concentrations in darker pigmented seeds compared to lighter pigmented seeds. Due to its position in the ABP preceding the production of these compounds, CHI has been chosen as the candidate gene target. My strategy is to sequence DNA from seed coats to look for mutations in CHI. Based on the literature I will likely find mutations that creates a premature stop or an insertion in the gene that decrease CHI’s function. However, while there are many studies in Soybean and Chickpea, there are relatively few studies on Lupin. This is critical work because of the ability to apply the results to agricultural and restoration. Additionally, this work adds to the novelty of the Anthocyanin Biosynthetic Pathway.

1 - Bowling Green, OH 43403, Bowling Green, OH, 43402, United States
2 - Bowling Green State University, Department Of Biological Sciences, Life Science Building, Bowling Green, OH, 43403, United States

Keywords:
polymorphisms
Anthocyanin
Biochemistry.

Presentation Type: Poster
Session: P, Functional Genetics and Genomics
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCG003
Abstract ID:203
Candidate for Awards:Genetics Section Poster Award