Abstract Detail

Molecular Ecology

Thapa, Ramhari [1], Bayer, Randall [2], Mandel, Jennifer [3].

The Development and Use of Microsatellite Markers for Investigating Clonal Diversity and Parental Origins in Complex Polyploid Species.

Antennaria (Asteraceae: Gnaphalieae) is a genus of dioecious, perennial herbs widely distributed in the Holarctic region with only three species reported in the Andes in South America. The genus comprises 34 known amphimictic (sexually reproducing) diploid/tetraploid species and more than five polyploid agamic (asexually seed producing) complexes. Antennaria rosea is one of the most morphologically diverse, polyploid agamic complexes in the genus with the cytotypes occurring as triploid (2n=42), tetraploid (2n=56), and pentaploid (2n=70). Morphological and isozyme studies show that the species is a compilospecies originated through multiple hybridization and introgression from among several species with morphology favoring the involvement of A. aromatica, A. corymbosa, A. marginata, A. microphylla, A. racemosa, A. rosulata, A. pulchella, and A. umbrinella in the parentage. Based on the possibility of variation searched in the alignment of next generation sequencing data targeting 1061 Conserved Orthologous Set (COS) loci earlier used for the reconstruction of the phylogeny of the genus, as well as, SSR enriched sequence data, Candidate Microsatellite (SSR) loci were identified for the genus using Phobos Software. In total, 36 primer pairs were developed using Primer3 software; of which, 26 loci amplified well, and variability among species and populations was seen in 19 loci. Measurement of the clonal diversity, investigation of parental origins, and determination of population genetic structure in 20 populations of A. rosea species collected from the major portion of the principal range of the species distribution in western North America was performed. The developed SSR markers will also be useful for population genetic studies in other diploid Antennaria species, as well as, in the remaining four polyploidy agamic complexes. As the SSR loci, especially developed from the COS loci, are selected from the conserved regions, the developed primers may also be transferable to closely related genera. In fact, a few of these loci successfully amplified in Chresta species from the Vernonieae tribe of the Asteraceae family.

1 - The University of Memphis, Department of Biological Sciences, 3700 Walker Avenue , Memphis, TN, 38152, USA
2 - University Of Memphis, Department Of Biological Sciences, 3700 Walker Avenue, 201 Ellington Hall, Memphis, TN, 38152, United States
3 - University of Memphis, 3700 Walker Avenue, Memphis, TN, 38152, United States

Keywords:
Antennaria rosea
Microsatellite
Genetic diversity.

Presentation Type: Oral Paper
Session: 22, Molecular Ecology
Location: 107/Mayo Civic Center
Date: Tuesday, July 24th, 2018
Time: 11:30 AM
Number: 22013
Abstract ID:202
Candidate for Awards:None