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Abstract Detail



Symbioses: Plant, Animal, and Microbe Interactions

Saba, Malka [1].

Diversity and community structure of Ectomycorrhizal fungi associated with Pinus roxburghii and P. wallichiana across the western Himalayan forests of Pakistan.

Diversity of ECM fungi were investigated both above-ground and below-ground associated with Pinus roxburghii and P. wallichiana growing in pure stands situated in western Himalayan region of Pakistan. We used direct sequencing of internal transcribed spacer region of nrDNA for the identification of sporocarps and ECM root tips. We have detected fifty fruit bodies and fifty six operational taxonomic units. The dominant above-ground fungus identified was Russula in association with Pinus roxburghii followed by Astraeus and Inocybe while Inocybe was the dominant genus associated with P. wallichiana followed by Russula and Lactarius. Inspite of several common taxa, Cortinarius species were exclusively found in association with P. wallichiana. Amongst the Operational Taxonomic Units (OTUs), members of the lineage russula-lactarius were found dominant in association with both host trees. Following are commonly associated ECM genera with both Pinus species; Amphinema, Inocybe, Lactarius, Russula, Sebacina and Tomentella. Inspite of these common taxa, Cenococcum, Helvellosebacina and Gymnomyces species were specifically found in association with P. roxburghii and Suillus and Tuber species were identified exclusively in association with P. wallichiana. Percent of taxa calculated from representative taxonomic groups in both above- and below-ground survey indicate the dominance of members of Inocybaceae aboveground while members of Russulaceae were abundant belowground. Taxa of Inocybaceae, Russulaceae and Suillaceae were collected during both above- and below-ground investigation. Different diversity patterns and fungal species composition identified in association with both host trees suggests that in addition to the spatial factors, host species also plays important role in structuring the ECM fungal community.


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1 - University of Gujrat, Botany, Hafiz Hayat Campus, Gujrat, Punjab, 50700, Pakistan

Keywords:
ECM fungi
nrDNA
Pinus roxburghii
P. wallichiana
Western Himalaya.

Presentation Type: Oral Paper
Session: 35, Symbioses: Plant, Animal, and Microbe Interactions
Location: 104/Mayo Civic Center
Date: Wednesday, July 25th, 2018
Time: 8:30 AM
Number: 35003
Abstract ID:187
Candidate for Awards:None


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