| Abstract Detail
Anatomy and Morphology Ahl, Louise [1], Grace, Olwen [2], Mravec, Jozef [3], Jørgensen, Bodil [4], Willats, William [5], Rønsted, Nina [1]. Analyses of Aloe polysaccharides using carbohydrate microarray profiling. As the popularity of Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another and the effects they have on the human body are increasing. Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of Aloe vera, and they have been widely studied in the past decades. However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions as most of them require a complete or near-complete fractionation of the polymers. The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in Aloe leaf mesophyll. We used Comprehensive Microarray Polymer Profiling (CoMPP) to investigate the polysaccharide compositions. This method combines the high-throughput capacity of microarray technology with the specificity of molecular probes. Our findings showed that CoMPP can successfully be used for high-throughput screening of Aloe leaf mesophyll tissue. A small selection of Aloe and closely related genera were analysed and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen. These data show that the polysaccharides vary between species, and that true species of Aloe differ from segregate genera. Log in to add this item to your schedule
1 - University of Copenhagen, Natural History Museum of Denmark, Øster Farimagsgade 5, Copenhagen, Denmark 2 - Royal Botanic Gardens Kew 3 - University of Copenhagen, Section for Plant Glycobiology, Thorvaldsensvej 40, Frederiksberg C, 1871, Denmark 4 - University of Copenhagen 5 - University of Newcastle
Keywords: Aloe Polysaccharides Succulence Succulent tissue CoMPP.
Presentation Type: Poster Session: P, Anatomy and Morphology Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center Date: Monday, July 23rd, 2018 Time: 5:30 PM This poster will be presented at 5:30 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm. Number: PAM003 Abstract ID:168 Candidate for Awards:Developmental and Structural Section best poster |