Abstract Detail

Biogeography

Knox, Eric [1], Case, Andrea [2], Appiah-Madson, Hannah [3], Caruso, Christina [4].

Post-glacial colonization of the Midwest by Lobelia siphilitica.

Plastid genomes are typically inherited uniparentally (usually maternally) and do not recombine, which makes plastid DNA-based phylogenetic estimates essentially matrilineal gene genealogies. DNA sequencing of Lobelia siphilitica collected near Yellowwood State Forest (Brown County, IN) revealed an unusual situation in which seed collected from a single plant (which are minimally maternal half-siblings) grew into plants that possessed two markedly different plastid haplotypes that were equally divergent from the inferred ancestral DNA sequence. Genome sequencing demonstrates that the western variety ludoviciana is sister to the eastern var. siphilitica, and a sample of the named hybrid L. × speciosa has var. siphilitica as the maternal parent and L. cardinalis as the paternal parent. The most rapidly evolving region is in and around a foreign gene of unknown function (ORF262) inserted between trnQ and rps16, and 580 plants from 86 Midwestern populations have been genotyped for this region. A 49-bp minisatellite repeat array upstream of ORF262 is hypervariable and 121 haplotypes have been discovered. Phylogenetic analysis demonstrates that multiple lineages survived the glacial maxima, and biogeographic analysis indicates that most terminal variation is associated with post-glacial colonization. Long-distance seed dispersal has been common during the past 10,000 years, but individual populations tend to be inbred with one or a few haplotypes that can be quite different from nearby populations.

1 - Indiana University, Department Of Biology, Bloomington, IN, 47405.0, United States
2 - Box 5190, 256 Cunningham Hall, Kent, OH, 44242, United States
3 - Northeastern University, Marine Science Center, Nahant, MA, 01908, USA
4 - University Of Guelph, Department Of Integrative Biology, Guelph, ON, N1G 2W1, Canada

Keywords:
Lobelia
Phylogenomics
plastome
Biogeography.

Presentation Type: Oral Paper
Session: 4, Biogeography
Location: 105/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 8:00 AM
Number: 4001
Abstract ID:156
Candidate for Awards:None