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Abstract Detail



Recent Topics Posters

Fisher, Hannah [1], Brown, Gregory [2], Evans, Timothy [3].

Evolution of PEP carboxykinase, an enzyme in the CAM photosynthetic pathway, in Bromelioideae (Bromeliaceae).

CAM photosynthesis is a pathway in which plants use an alternative mechanism for carbon fixation than that used in the typical C3 photosynthesis. This pathway has been derived several times within Bromeliaceae subfamily Bromelioideae. In this study, we examined the evolution and predicted protein structure of PEP carboxykinase (PEPCK), an enzyme in the CAM photosynthetic pathway. Exon regions in the gene that codes for PEPCK were sequenced in 9 species from subfamily Bromelioideae representing both CAM and C3 lineages, and the inferred amino acid sequence for each species was determined. The 3-dimensional protein structure was predicted for each species based on the amino acid sequences and compared to one another. Variation was found within both the DNA and inferred amino acid sequences. Preliminary analyses have also revealed variation in protein tertiary structure within and between CAM and C3 species. This variation will be interpreted in the context of enzyme function and phylogenetic structure within Bromelioideae.


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1 - Grand Valley State University, Biology, 1 Campus Drive, Allendale, MI, 49401, USA
2 - UNIVERSITY OF WYOMING, Department Of Botany, Box 3165, Laramie, WY, 82071, United States
3 - Grand Valley State University, Biology, 1 Campus Drive, 3321 Kindschi, Allendale, MI, 49401, United States

Keywords:
CAM photosynthesis
Bromeliaceae
PEP carboxykinase
Bromelioideae.

Presentation Type: Recent Topics Poster
Session: P, Recent Topics Posters
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM
Number: PRT023
Abstract ID:1318
Candidate for Awards:None


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