| Abstract Detail
Comparative Genomics/Transcriptomics Catlin, Nathan [1], Wing, Rod A. [2], Soltis, Douglas [3], Soltis, Pamela S. [4], Barbazuk, W. Brad [1]. The interplay of alternative splicing and gene duplication in Oryza. Large, clade-wide sequencing projects allow for genomic and transcriptomic-level comparisons across taxa, which provide an evolutionary context for various genetic mechanisms. Two such mechanisms are alternative splicing (AS) and gene duplication. AS is a pre-mRNA processing mechanism resulting in the production of multiple mRNA transcripts from a single gene. Gene duplication, alternatively, is a genomic structural change resulting in an immediate increase in genetic material. Therefore, gene duplication is often seen as a possible mode of functional divergence and diversification. These processes are known to play crucial roles in many important plant life functions, including floral development, grain quality, and stress responses. Although it is known that AS and gene duplication are ubiquitous across plant taxa, the evolution and relationship between the two are a not clearly understood. The rice genus (Oryza) provides an ideal system for tandemly investigating AS and gene duplication in an evolutionary framework due to the enormous amount of sequencing data available for domesticated rice and its wild relatives. By leveraging public RNA-Seq data and genomes for 9 species of Oryza, I have characterized the variation of AS across the rice genus, Oryza. Additionally, AS can be linked to each generated orthogroup phylogeny within Oryza, allowing for the first direct comparison of AS events and gene duplication on clade by clade basis within a rapidly diverging genus.
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1 - University of Florida, Biology, 220 Bartram Hall , P.O. Box 118525 , Gainesville, FL, 32611-8525, USA
2 - Arizona Genomics Institute, School of Plant Sciences, University of Arizona, Tucson, AZ, USA
3 - University of Florida, Biology, Gainesville, FL
4 - University of Florida, Florida Museum of Natural History, 1659 Museum Road, Gainesville, FL, 32611, US
Keywords:
Alternative splicing
gene duplication
Oryza
transcriptomics.
Presentation Type: Oral Paper
Session: 25, Comparative Genomics and Transcriptomics II
Location: 102/Mayo Civic Center
Date: Tuesday, July 24th, 2018
Time: 5:00 PM
Number: 25016
Abstract ID:941
Candidate for Awards:Margaret Menzel Award |
